Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
Tris-Buffered Saline 10X pH 7.6 (BM-615): 250 mM Tris, 27 mM KCl, 1.37 M NaCl, pH 7.6 +/- 0.2. Prepared in 18.2 megohms water. To make 1X, dilute 10-fold with deionized water. The pH of the 1X should be 7.6 +/- 0.2.
CHEMSCIENCE Soluble Sulfate for SO3
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
Kd Medical Inc TRIS GLYC SDS BUFFER, 10X, 4L
10X Tris Glycine SDS is used for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. One 4 liter cubitainer.
LAB BIOREAGENTS LowBinding I Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
TARGETMOL CHEMICALS INC NVP-TAE 226 5MG
Also available in 1 mL, 1 mg, 2 mg, 10 mg, 25 mg, 50 mg, 100 mg and bulk. Please contact Fisher for quotes. NVP-TAE 226 (TAE226) is a potent FAK inhibitor (IC50 5.5 nM), exhibiting even greater efficacy against Pyk2 (IC50 3.5 nM), and is 10- to 100-fold less effective against IGF-1R, InsR, c-Met, and ALK. Purity 98.07%
Crescent Chemical Co Inc EDTA ANALYTICAL GRADE
Ethylenediamine tetraacetic acid analytical grade Size - 100G Storage Conditions - +15 °C TO +30 °C Catalog Number - 11278.01 CAS 60-00-4
Crescent Chemical Co Inc TRIS(HYDROXYMETHYL)METHYLGLYCI
Tris(hydroxymethyl)methylglycine analytical grade MP 179 - 187 °C; pKa 20 = 8.15. Buffering substance (1). Non-toxic substitute for barbital (2). Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 37195.03 CAS 1389-47-5
Crescent Chemical Co Inc PROTEIN TEST MIX 4 FOR SDS PG
Protein Test Mixture 4 for SDS PAGE 4 Dalton-marker proteins, high molecular weight, for SDS PAGE Molecular weight markers for SDS gel electrophoresis. The bovine albumin (BSA) contains monomers and oligomers.Reconstitute with SDS sample buffer (125 mM Tris-HCl pH 6.8, 2 % SDS, 15 %