Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-1000.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 1 Kilogram
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 7.5) - 250ml
Bicine Buffer (0 5 M pH 7 5) - 250ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
Teknova 1M HEPES Solution, pH 6.5. 1000 mL, Sterile.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255318.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Dojindo Molecular Technologies Inc MOPS (25G), Good's buffer, CAS: 1132-61-2, pka=7.2, Active pH 6.5-7.9, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid | 4432-31-9 | 554642 | 99.9% | 500 MG
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity: pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Applications include cell culture, enzyme activity determination, electrophoresis, and protein studies
eMolecules TES | 7365-44-8 | MFCD00007532 | 1g
Oakwood Chemical | TES | 1g | 537724648 | M05749 | | 7365-44-8 | MFCD00007532 | 229.250 | C6H15NO6S
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Bioworld ACES Buffer, 0.5M, pH 7.5, 500 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 6.5, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltpH: useful range: 6.0 - 7.2Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. PH Value: : 6.0 - 7.2
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
Research Products International Corp PIPES Sesquisodium Salt 5 KG
PIPES Sesquisodium Salt, also known as PIPES Na3, is a chemical compound that has significant applications in the life science marketplace. PIPES stands for piperazine-N,N'-bis(2-ethanesulfonic acid), and the sesquisodium salt refers to the trisodium salt form of PIPES.
It is primarily utilized as a buffering agent. Buffers are substances that help maintain a stable pH in biological and biochemical processes, ensuring optimal conditions for various enzymatic reactions and cellular activities.
PIPES Sesquisodium Salt is particularly valuable in biological research, molecular biology, and cell culture applications. It offers excellent buffering capacity in the physiological pH range (6.1 - 7.5), making it suitable for a wide range of experimental conditions.